【摘要】 目的 :研究 2 3 羟基桦木酸在体外对B16、SGC 790 1、A0、HL 60、K5 62、HeLa及S180 7种恶性肿瘤细胞生长的抑制作用。方法 :应用四甲基偶氮唑盐比色分析、电镜观察、流式细胞术等方法观测 2 3 羟基桦木酸对 7种肿瘤细胞的抑制作用。结果 :10～ 160mg·L-1的 2 3 羟基桦木酸在体外对 7种肿瘤细胞有显著的抑制作用 ,呈一定的剂量依赖关系 ,并可引起大量肿瘤细胞凋亡。 2 3 羟基桦木酸 Ⅰ使B16肿瘤细胞被阻滞在G0 G1期 ,而 2 3 羟基桦木酸 Ⅱ则使细胞阻滞在G2 M期。结论 :2 3 羟基桦木酸对体外 7种恶性肿瘤细胞均有显著的抑制作用 ,并使细胞阻滞在某一生长期 ,最终导致肿瘤细胞凋亡。更多还原

【Abstract】 Objective To study the growth inhibition in seven cancer cell lines induced by 23?hydroxyl betulinic acid in vitro .Methods 3?(4,5?dimethylthiazole?2?yl)?2,5?diphenyl?tetrazolium bromide(MTT) assay,electronic microscopy and flow cytometry were used to examine the effect of 23?hydroxyl betulinic acid on seven cancer cell lines.Results 23?hydroxyl betulinic acid at the dose of 10～160 mg·L -1 markedly inhibited the growth of seven cancer cell lines in dose dependent man ner,and finally cause a great number of cancer cells apoptosis.23?hydroxyl betulinic acid?Ⅰ result in accumulation of B16 cells by retarding cell cycle at G 0?G 1 phase and 23?hydroxyl betulinic acid?Ⅱ arresting S180 at G 2?M phase.Conclusions 23?hydroxyl betulinic acid significantly inhibited the proliferation of seven cancer cell lines by retarding cells at certain phase of cell cycle and finally caused cells apoptosis.更多还原