【摘要】 用RT PCR技术、PCR标记的探针点杂交和SDS PAGE检测了生产上严重危害玉米和水稻的水稻黑条矮缩病毒(RBSDV)。由RT PCR扩增的RBSDV第 7片段第 92 1～ 14 11碱基作探针 ,用PCR法DIG标记后点杂交 ,可以从 10 0ng玉米病叶中检测到RBSDV ,灵敏度是RT PCR的 1/10 ;10 %SDS PAGE只能检测到从 1g玉米病叶中提取的病毒dsRNA ,但对江苏玉米田间分离的不同的RBSDV样品电泳发现 ,该病毒基因组dsRNA有差异 ,表明该技术是研究RBSDV基因组多样性的简单有效的方法更多还原

【Abstract】 The purpose of this study was evaluation of RT PCR technique,dot blot hybridization with PCR generated probe and SDS PAGE for the detection of rice black streaked dwarf Fijivirus(RBSDV).The cDNA probe labeled with digoxigenin by PCR,complementary to the region between 921 and 1 411 nucleotides of segment 7 of RBSDV from Jiangsu,was used.In dot blot hybridization the probe could detect the viral RNA from 100 ng diseased maize leaves,which is ten times less sensitive than RT PCR.10% SDS PAGE only could detect the viral dsRNA extracted from 1 g diseased maize leaves,but this technique was found to be simple and effective to study the genomic diversity of RBSDV isolated in fields.更多还原