【摘要】 测定了高羊茅 (FestucaarundinaceaSchreb .)和草地早熟禾 (PoapratensisL .)幼苗地上部组织超氧化物歧化酶(SOD)、过氧化物酶 (POD)、抗坏血酸过氧化物酶 (APX)和过氧化氢酶 (CAT) 4种保护酶的最适pH。结果两者幼苗 :APX和CAT的最适pH相同 ,分别为在醋酸缓冲液中pH 5 6和在磷酸缓冲液 (PBS)中pH 7 4 ;但SOD和POD的最适pH不同 ,高羊茅地上部SOD和POD的最适pH在PBS中分别为 7 8和 6 0 ,而草地早熟禾SOD在PBS中的最适pH为 7 8,在Tris HCl缓冲液中的最适pH为 8 2 ,其POD的最适pH在醋酸缓冲液中为 5 0。更多还原

【Abstract】 Optimal pH for 4 antioxidant enzymes,superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX)and catalase(CAT),was investigated in the seedlings of tall fescue(Festuca arundinacea Schreb.)and Kentucky bluegrass(Poa pratensis L.).The optimal pH levels for APX and CAT are the same at 5 6 in HAc NaAc buffer and 7 4 in sodium phosphate buffer(PBS),respectively,in tall fescue and Kentucky bluegrass.Yet the optimal pH for SOD and POD are different between the two grasses.It is at 7 8 and 6 0 in PBS for SOD and POD,respectively,in tall fescue.However the optimum pH for SOD is at 7 8 in PBS or 8 2 in Tris HCl buffer and for POD is 5 0 in HAc NaAc buffer in Kentucky bluegrass.更多还原