【摘要】 实验表明T .ferrooxidans对FeSO4和硫等单或双底物利用的难易程度次序为 :单底物培养基中FeSO4最易利用 ,Na2 S2 O3 其次 ,单质硫最难利用 ;双底物培养基中 ,T .Ferrooxidans首先利用Fe2 + ,然后利用硫 ,此外培养液中还伴随发生化学氧化反应、中和沉淀、生物氧化等反应 ,以上反应使培养液中亚铁的浓度在培养中期上下波动直至铁氧化酶抑制被解除。更多还原

【Abstract】 Experiments revealed that the energy source utilization order of T. ferrooxidans in the single or double substrates systems as follows:the ferrous salt was firstly easy to use, Na 2S 2O 3 secondly easy and sulfur the last in the single substrates system. In the double substrates broth,the ferro oxidation enzyme of T. ferrooxidans firstly initiate the energy source utilization, two enzyme systems of T. ferrooxidans worked simultaneously as the concentration of Fe 2+ lowers down. In the double substrate broth, the bio oxidation , the chemical oxidation and deposition occurred simultaneously too. Competition of above reactions caused the concentration of the ferrous ions keeping fluctuation for a long time in the middle pieod of the cultivation of T. Ferrooxidans ,until pH declined enough and the ferro oxidation enzyme relived from repression.更多还原