【摘要】 目的 :通过建立兔放射性脑损伤模型 ,研究IL 8单抗对放射性脑损伤的作用及其机理。方法 :30只新西兰兔行脊髓蛛网膜下腔置管 ,分成二组 ,照射前 30分钟和照射后六天内每天向蛛网膜下腔注入IL 8单抗或生理盐水 ,分别在 7、14、30天处死取样 ,行病理免疫组化和脑脊液IL 8水平检测。结果 :治疗组与对照组各时间段相比 ,放射性脑损伤的病理形态学改变减轻。IL 8浓度明显降低 (P <0 0 0 1) ,胶质细胞原纤维酸性蛋白 (GFAP)、脑一氧化氮合成酶 (bNOS)阳性细胞数较NS组显著减少。结论 :10 0Gy剂量能造成放射性脑损伤 ,蛛网膜下腔注入IL 8单抗对放射性脑损伤具有保护作用更多还原

【Abstract】 Objective:To study the effect of IL-8Ab administration in a rabbit model of radiation injury.Methods:The rabbit model was established by putting a pipe into spine subarachnoid.Thirty rabbits were divided into two experimental groups before undergoing radiosurgery:NS(0.1ml/kg n=15);IL-8m Ab(10μg/kg n=15).The drug was administered 30 minutes before radiosurgery and six days after radiosurgery.Radiosurgery(Co 60 2×2cm 100Gy)of the normal rabbit frontal lobe was performed.All brains underwent histological|electromicroscope、immunohistological examination and IL-8 detection in cerebrospinal fluid.Result:Morphologioal and eletromicroscope results showed IL-8mAb can reduce edema in the lesion tissue.IL-8 level of IL-8mAb group in cerebrospinal fluid was significant decreased than the control(P<0.001).The number of GFAP and bNOS positive cell of IL-8Ab group also lessened than the control.Conclusion:Investigation indicated that the NS group had severe injury and IL-8mAb provided protection after delivering drug through arachnoid against radiation-induced injury.\;更多还原