【摘要】 目的 :观察正常大鼠脑受γ 刀照射后 ,热休克蛋白 70 (ＨＳＰ70 )在神经元、神经胶质细胞和血管内皮细胞的表达及变化。方法 :45只正常成年大鼠脑接受γ 刀 1 0 0Ｇｙ量的照射 ,分别成活 0 5ｈ、1ｈ、3ｈ、6ｈ、1 2ｈ、1ｄ、3ｄ、7ｄ、1 4ｄ、3 0ｄ和 3个月后被处死 ,固定取脑 ,切制冰冻切片 ,进行抗ＨＳＰ70的免疫组织化学反应。结果 :血管最先出现反应 ,成血管扩张 ,内皮细胞成ＨＳＰ70阳性反应 ;其次白质内胶质细胞出现ＨＳＰ70阳性反应 ;皮质等处神经元较晚 (3ｄ起 )出现明显的反应。上述阳性反应又显示出不同的变化规律。结论 :结果表明γ 刀照射虽然局限于一点 ,但其反应是广泛的。更多还原

【Abstract】 Objective:To diserve the expression and change of heat shock protein 70(HSP70)in neurons,glia cells and endothalial cells of normal rat brain after γ-knife irradiation.Method:Forty-five normal aduct rat brain received 100 Gy dosage of γ-knife irradiaation.Method:Forty-five normal aduct rat brain received 100 Gy dosage of γ-knife irradiating and sunived respectively for 0.5h,1h,3h,6h,12h,1d,3d,7d,14d,30d and 3 months.The animals were killed and perfused with fixative,the brain were removed,cut into frozen sections and anti HSP70 immunohistochemical reactions were done.Result:The results were that firstly,the blood vessels were enlarged and its endothelial cells were HSP70 positive,then the glia cells in the white matter related with the lateral ventricle exhibited HSP70 positive,and the neurons in the cortex etc,appeared evident HSP70 reactions relatively late (from 3d).Different ways of changes were found in the above positive reactions.Conclusion:The present results suggest that although irradiation of gamma knife was locadized in one point but the response to irradiation occurred exteusively.\;更多还原