【摘要】 对几种产L -乳酸的根霉菌进行了筛选 ,根据其产酸水平及稳定性筛选出一株高产菌 ,并就其发酵条件对产酸水平的影响作了初步探讨 从 5株购得菌中筛选出了少根根霉As3.2 893,并确定发酵条件如下 :氮源采用硫酸铵 ,浓度为 0 .6% ,装液量 4 0～ 5 0mL/ 2 5 0mL锥形瓶 ,发酵温度 30℃ ,发酵时间 4 8h 在摇瓶培养条件下 ,其产酸水平稳定在 5 .8～ 6.5 g/ 10 0mL更多还原

【Abstract】 This paper was to describe the selection of Rhizopus Sp.producing L-lactic acid.And the fermentation factors influencing acid production were investigated.According to the yield of the L-lactic acid and the stability,the Rhizopus arrhizus As 3.2893 was selected from five Rhizopus Sp..And its culture condition was determined as follows:0.6% (NH 4) 2SO 4,liquid level 40～50 mL/250 mL flask,fermentation temperature 30 ℃,fermentation time 48 h.In shaking flask culture,lactic acid yields of As 3.2893 were 5.8～6.5 g/100 mL.更多还原