【摘要】 目的：探讨胃肠道恶性肿瘤及其切缘组织中ｃ－ｍｙｃ基因ｍＲＮＡ和端粒酶活性表达与手术切缘的安全性关系，方法：用 ＲＴ－ＰＣＲ和 ＴＲＡＰ方法检测 １２例胃癌和 １８例肠癌标本和切缘组织中ｃ－ｍｙｃ基因ｍＲＮＡ和端粒酶活性的表达．结果：（ｌ）胃肠道恶性肿瘤组织中ｃ－ｍｙｃ基因ｍＲＮＡ和端粒酶活性有很高的表达率．（２）肿瘤的上下切缘组织中ｃ－ｍｙｃ基因ｍＲＮＡ和端粒酶活性表达无明显差异．（３）在中心表达阳性的肿瘤中，距中心４ｃｍ以上端粒酶均为阴性表达，而 ｃ－ｍｙｃ基因 ｍＲＮＡ在距 ４ｃｍ处仍有小部分呈阳性表达， ５ｃｍ处则呈阴性，结论：就 ｃ－ｍｙｃ基因 ｍＲＮＡ表达和端粒酶活性表达而言，距离肿瘤 ５ｃｍ可定为肿瘤的分子切缘．更多还原

【Abstract】 Objective: To detect the c-myc mRNA and telomerase activity(TA) expression in the tumor center and peripheral tissues of patients with gastrointestinal cancers, and to explore the security of tumor surgical margins. Methods: To detect the expression of c-myc mRNA and TA in tumor tissues,the approximal and distal peripheral tissues, every lcm away from the tumor margin of 12 gastric and 18 colorectal carcinomas with RT-PCR and TRAP methods respectively. Results: (l)The expression rates of c-myc mRNA and TA in tumor tissues are high. (2) There are no remarkable differences of c-myc mRNA and TA expressions between higher or lower tumor surgical margins. (3) C-myc mRNA and TA can’t be detected in 5-cm away peripheral tissues. Conclusion: Considering C-myc mRNA and TA expression, 5 cm can be defined as molecular margin.更多还原