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猪细小病毒VP2基因的克隆与序列测定
Cloning and sequencing of VP2 gene of PPV BM-1 strain
【摘要】 本研究以PCR方法成功扩增并克隆了BM-1株PPV结构蛋的VP2基因的上、下游片段。通过VP2基因的序列测定,发现我国BM-1 PPV VP2基因序列与国外发表的序列(NADL-2、Kresse等)的有一定差异。
【Abstract】 Porcine parvovirus (PPV) BM-1 strain Vp2 gene was amplified by polyme rase chain reaction with two pair of primers and two fragments. The sequences we re determined after these fragments being cloned by pGEM T vector. It was shown that the BM-1 strain PPV VP2 was different from NADL-2 in sequence analysis.
【关键词】 猪细小病毒;
VP2基因;
克隆;
序列测定;
【Key words】 porcine parvovirus; Vp2 gene; cloning; sequence determination;
【Key words】 porcine parvovirus; Vp2 gene; cloning; sequence determination;
- 【文献出处】 郑州牧业工程高等专科学校学报 ,Journal of Zhengzhou College of Animal Husbandry Engineering , 编辑部邮箱 ,2001年03期
- 【分类号】S858.28
- 【被引频次】4
- 【下载频次】83