【摘要】 目的 :探讨cyclinE和p2 1WAF1/CIP1蛋白在胃癌发生发展中的作用及其表达的意义。方法 :采用免疫组化S P法检测正常胃黏膜、萎缩性胃炎伴肠上皮化生、萎缩性胃炎伴不典型增生各 2 0例和 78例胃腺癌组织中cyclinE和 p2 1WAF1/CIP1蛋白表达。结果 :cyclinE蛋白阳性表达在胃癌组高于正常胃黏膜、萎缩性胃炎伴肠上皮化生组 ,而 p2 1WAF1/CIP1蛋白表达则相反 ,差异均有显著性 (P <0 0 5 ) ;cyclinE、p2 1WAF1/CIP1蛋白表达与胃癌细胞分化程度相关 (P <0 0 5 ) ;有肝转移的胃癌组cyclinE阳性表达率高于无肝转移组 (P <0 0 5 ) ;有淋巴结转移组 p2 1WAF1/CIP1蛋白表达率低于无淋巴结转移组 (P <0 0 5 )。结论 :cyclinE蛋白高表达与 p2 1WAF1/CIP1蛋白失表达可能参与胃癌的发生发展过程 ,检测cyclinE和p2 1WAF1/CIP1蛋白作为反映胃癌病理学特点的参考指标可能有一定意义更多还原

【Abstract】 Purpose To investigate the cyclinE and p21 WAF1/CIP1 protein in gastric carcinogenesis and the significance of their expression in gastric carcinoma(GC). Method Twenty samples of normal gastric mucosa, 20 samples chronic atrophic gastritis (CAG) with metaplasia, 20 samples CAG with dysplasia and 78 cases of GC were examined by immunohistochemical method. Results The positive rate of cyclinE was higher but p21 WAF1/CIP1 was lower in GC than that in normal gastric mucosa and CAG with metaplasis( P< 0 05). The expression of cyclinE and p21 WAF1/CIP1 in GC significantly correlated with tumor cell differentiation( P< 0 01). The positive rate of cyclinE was higher in GC with liver metastasis group than that without live metastasis one( P< 0 05). The positive rate of p21 WAF1/CIP1 protein expression was lower in GC with lymph node metastasis group than that without node involovement one ( P< 0 05). Conclusions The high expression of cyclinE and the lose of p21 WAF1/CIP1 expression may be associated with gastric carcinogenesis. The detection for cyclinE and p21 WAF1/CIP1 protein may be helpful to evaluate the clinicopathological parameters of gastric carcinoma.更多还原