【摘要】 目的 :揭示溃疡性结肠炎 (UC)结肠黏膜肿瘤坏死因子 α(TNF α)的表达以及与溃疡性结肠炎不同时期浸润的炎细胞免疫表型之间的关系。方法 :应用抗T淋巴细胞 (CD45RO)、抗B淋巴细胞 (CD2 0 )、抗巨噬细胞 (CD68)表面抗原的单克隆抗体 ,对 5 0例溃疡性结肠炎结肠黏膜活检标本 (32例活动期 ,18例非活动期 )及 5例癌旁正常组织作为对照进行了淋巴细胞亚型分析 ,应用原位杂交技术 ,对上述病例进行了TNF α的检测分析。结果 :T淋巴细胞数量在活动期高于非活动期及对照组 (P <0 0 1)。而固有层内B淋巴细胞数量在三组之间差异无显著性。巨噬细胞的数量在活动期高于非活动期及正常对照组 (P <0 0 1)。细胞因子TNF αmRNA的表达在活动期高于非活动期。结论 :TNF α在溃疡性结肠炎发病机制中起到了重要作用。其可作为炎症介质 ,介导结肠黏膜的病理性损伤。更多还原

【Abstract】 Purpose To explore the expression of tumor necrosis factor α (TNF α) and its relationship with different leucocyte subsets in ulcerative colitis(UC). Methods Immunohistochemistry and in situ hybridization techniques were used to detect expression of different leucocyte subsets and TNF α mRNA in biopsy specimens of the colonic mucosa from 50 patients with UC and 5 cases of normal control. Results The number of CD45RO T lymphocytes was significantly higher in active UC ( n =32) than that in inactive UC ( n =18) or normal control ( n =5). In comparison to controls, there was no difference of CD20 B lymphocytes in lamina propria among the three groups. Significant difference in macrophages was found between the patients with active UC and ones with inactive UC ( P <0 01). TNF α mRNA expression was significantly increased in active UC than in inactive UC ( P <0 01). Conclusions TNF α expression might play an important role in pathogene of UC. Inflammatory factors lead to tissue destruction of UC.更多还原