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不夜城的快速繁殖

Rapid Clonal Propagation of Alon nobilis

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【作者】 韩阳娄和林赵彦芳闫秀丽简弘晨

【Author】 HAN Yang 1,LOU Helin 1,ZHAO Yanfang 1,YAN Xiuli 2, JIAN Hongchen 1 1.College of Environment and Life Science, Liaoning University 110036; 2.Shenyang Aeronautical Engineering Institute, Shenyang 110034

【机构】 辽宁大学环境与生命科学学院!辽宁沈阳110036沈阳航空学院!辽宁沈阳110034

【摘要】 以不夜城幼茎为材料 ,经表面消毒后接种于诱导培养基上 ,4周左右出现不定芽 .不定芽在壮苗培养基上培养 2周转至生根培养基上 ,又经 3周长出完整根系 .再生植株炼苗后移栽到基质中 ,移栽成活率为 90 % .

【Abstract】 The young stems of Aoln nobili were used as explant material and cultured on the inducing media. The results showed that a number of adventitious shoots grew on the surface of explant after 4 weeks of inoculation. Many adventitious shoots were acquired through proliferating culture. After they have grown on nutritious media for 2 weeks, they were transferred on the root inducing media. Every shoots grew a number of roots and developed into a whole plantlet. The regenerated plantlets were replanted in sand, about 90 percent of the plantlets survived.

【关键词】 不夜城不定芽再生植株
【Key words】 Alon nobilisadventitious shootregenerated plantlet.
  • 【文献出处】 辽宁大学学报(自然科学版) ,