【摘要】 RNA是以磷酸二酯键相连的核苷酸组成的聚阴离子，而蛋白质在pH小于它的等电点的缓冲溶液中带有正电性。因此，它们在离子交换色谱分析时能出现在差异很大的洗脱组分中。据此，我们设计了用离子交换色谱法研究蛋白质与RNA的结合特异性方法，用以研究位于T细胞核内的具有RNA结合活性的蛋白质人亲环素33（hCyP33）与不同RNA的结合特异性。结果表明，人亲环素33只与有poly(A)尾序列结构的mRNA，即poly(A)+RNA发生特异性结合。更多还原

【Abstract】 RNA is polyanion which consists of nucleotides connected with phosphobiester bounds, whereas protein is positively loaded in the buffer which pH is lower than its isoelectric point. Therefore, they emerge in quite different eluted fractons by analysis of ionexchange chromatography. On this account we designed the method for investigation of the binding specificity of protein and RNA with ionexchange chromatography. In this article we investigate the binding specificity of human cyclophilin 33 (hCyP33), a RNA binding protein, with different RNAs. The result showes that human cyclophilin 33 binds specifically with poly(A) tailing mRNA, namely poly(A)+RNA.更多还原