【摘要】 [目的 ]探讨金属硫蛋白基因诱导表达能力与Ⅱ型糖尿病小鼠 (ob/ob小鼠 )对镉金属硫蛋白肾脏毒性易感性之间的关系。 [方法 ]以 β actin为内参照 ,采用半定量RT PCR方法检测在相同肾皮质镉水平条件下 ,镉对ob/ob小鼠和瘦型小鼠 (正常小鼠 )肾皮质MT1和MT2基因的诱导表达。 [结果 ]镉对ob/ob小鼠肾皮质MT1和MT2基因诱导表达的能力过低 ,而镉对瘦型小鼠MT1及MT2基因具有明显的诱导能力。 [结论 ]本研究提示Ⅱ型糖尿病小鼠对镉肾脏毒性易感的可能原因与糖尿病小鼠肾中镉对MT基因的诱导能力过低有关更多还原

【Abstract】 The aim of this study was to explore the ability of induced expression of metallothionein genes related to the susceptibility of type 2 diabetic mice (ob/ob mice) to cadmium metallothionein(CdMT) induced nephrotoxicity. With semi quantitative RT PCR,gene expressions of MT1 and MT2 induced by the same cadmium level in the renal cortex of type 2 diabetic mice and their lean litter mates (normal mice) were studied. Gene expressions of MT1 and MT2 in the renal cortex of ob/ob mice could not be induced obviously by CdMT. However they could be induced significantly in that of normal mice. [Conclusion] The authors concluded that there were some relationships between the possible mechanisms of the susceptibility of type 2 diabetic mice to CdMT induced nephrotoxicity and the lower ability of induced expressions of MT1 and MT2 genes in the renal cortex.更多还原