【摘要】 目的　研究γ-氨基丁酸 (GABA)转运体在点燃中的作用。方法　建立大鼠戊四氮点燃模型 ,采用逆转录聚合酶链式反应 (RT- PCR) ,半定量测定 GABA转运体亚型基因 (GAT- 1m RNA )的表达。结果　GAT- 1m RNA在点燃后即刻、48小时、7天均有高表达 ,30天后 ,GAT- 1m RNA的表达恢复到对照水平 ,6 0天后 ,GAT- 1m RNA的表达无变化。结论　 GAT- 1m RNA的表达上调 ,可能是点燃及癫痫敏感性形成的分子机制之一。更多还原

【Abstract】 Objective To study the role of GABA (γ aminobutyric acid) transporters in kindled.Methods The rat’s GABA model following PTZ kindled was made.The expression of GABA transporter subtype in the hippocampus(GAT 1 mRNA) was measured after PTZ (pentylenetetrazol) kindled seizures by using semiquantitative technique,reverse transcription polymerase chain reaction (RT PCR).Results GAT 1 mRNA was significantly increased in the hippocampus at 0 hour,48 hours,and 1 week after PTZ kindled seizures. Up regulation of expression of GAT 1 mRNA returned to the control levels after 1 month. No changes in the expression of GAT 1 mRNA were observed after 60 days of PTZ kindled seizures.Conclusion The expression of GAT 1 mRNA with up regulation may be associated with the epilepsy susceptibility.更多还原