【摘要】 目的 :研究口腔鳞癌、切缘组织和正常粘膜的DNA倍性特征、S期细胞比率 (S - phasefraction ,SPF)及临床意义。方法 :用FACS检测 39例患者口腔鳞癌、切缘组织及正常粘膜的DNA倍性并计算S期细胞比率。结果 :口腔鳞癌和切缘组织的异倍体率分别为 38%和 31% ,无统计学差异 ,但两者均显著高于正常口腔粘膜的异倍体率15 %。癌组织中异倍体患者的颈淋巴结转移率 40 % ,显著高于二倍体患者 2 1% (P <0 .0 2 5 )。切缘组织异倍体患者与二倍体患者的颈淋巴结转移无显著差异。口腔鳞癌与切缘组织间SPF分别为 2 3± 8.6 %、2 6± 9.2 % ,两者显著高于正常粘膜 12± 4.9% (P <0 .5 )。结论 :口腔鳞癌异倍体与颈淋巴结转移有关。切缘组织具有癌变的潜能更多还原

【Abstract】 Objective:To investigate DNA ploidy and S-phase fractio n of cancer nests ,the tissue of incisional edge and normal mucosa from patients with oral squamous cell carcinoma.Methods:DNA ploidy of oral squamous cell carcinoma,the tissue of incisional edge and oral mucosa obtained from 39 patients with oral squamous cell carcinoma were detected by FACS.S-phase fraction of them was calculated.Results:The aneuploidy rate of oral squamous cell carcinoma and the tissue of incisional edge was 38%(15/39) and 31%(12/39),respectively.There was no statistical difference between them in the aneuploidy rate.But the aneuploidy rate of two was significantly higher than those of oral mucosa 15%(6/39).The incidence of cervical lymph node metastasis was significantly (P<0.025)higher in the aneuploidy cases(6/15) than in the diploid cases(5/24) from cancer nests.There was no significant difference for the incidence of cervical lymph node metastasis between the aneuploidy cases and the diploid cases from the tissue of incisional edge.S-phase fraction of cancer nests and the tissue of incisional edge were 23±8.6%?26±9.2%,respectively.S-phase fraction was significantly higher in them than in oral mucosa 12±4.9 %(P<0.5).Conclusions:These results suggest a significant relationship between aneuploidy and incidence of the cervical lymph node metastasis.The tissue of incisional edge maybe have the potentiality of malignant transformation.更多还原