【摘要】 采用 SD大鼠 30只 ,2 5只为试验组 ,5只为对照组 .在三维立体定向仪上用微量注射器将6 - OHDA32 μg分两点注射入右侧纹状体 ,每周检测动物的旋转行为 ,第 1周动物没有出现旋转行为 ,第 2周动物出现明显的旋转行为 ,随后缓慢上升 ;至术后 5周才升至较高的水平并保持平稳 .其中有 15只动物达到或超过了 7r/ min这一通常采用的标准 ,模型成功率达 6 0 % .酪氨酸羟化酶免疫组化显示 :术后第 1周至第 5周右侧黑质 TH免疫反应细胞逐渐减少 ,各个时期的右侧黑质TH免疫反应细胞较对侧明显减少 .采用此方法建立的大鼠帕金森氏病模型更加符合临床 PD病人的病程进展并明显提高成功率更多还原

【Abstract】 Stereotaxic injection of 6 hydroxydopamine into the striatum of rats was performed to develop Parkinson`s disease models in thirty SD rats.One week after 6 OHDA injection into the striatum, no Apomorphine induced rotation was recorded, while obvious rotation appeared after two weeks, turns increased in the following 4 weeks, average 8.5 turns/min was recorded in fifth week and the rotation was stable in the following 1 month. The turns of 15 rats exceeded 7 turns/time.With the method of immunocytochemistry of Tyrosine hydroxylase(TH), the amount of TH immunoreactive(TH IR) neuronal cell numbers in the SN ipsilateral to the injection was decreased progressively,but no changes was seen in the right SN and in the control rats. These results suggested that the animal behavioral change after stereotaxic injection of 6 OHDA into striatum was much more like the progressive development of clinic PD patients.更多还原