【摘要】 探讨雄激素睾酮对雄性家兔腹主动脉球囊损伤后内膜增生的作用 ,选择健康雄性新西兰白兔 2 4只 ,随机分成对照组 (假去势 )、低睾酮血症组 (单纯去势 )和睾酮替代组 (去势一周后加用长效十一酸睾酮单次肌注 ,14mg kg) ,去势 10天后对所有动物行腹主动脉球囊损伤术。损伤后两周检测血浆睾酮、一氧化氮、超氧化物歧化酶及丙二醛水平 ,并截取腹主动脉进行图像和增殖细胞核抗原分析。结果发现 ,假去势对照组和睾酮替代组新生内膜面积和增殖细胞核抗原增殖指数明显低于低睾酮血症组 (P <0 .0 1) ,且血浆一氧化氮水平明显上升 ,丙二醛水平显著下降 (P <0 .0 1或 0 .0 5 )。结果提示 ,生理水平的睾酮 (内源或外源补充 )有抑制动脉损伤介导的内膜增生作用 ,其机制可能与睾酮增加一氧化氮的产生和抗氧化作用有关。更多还原

【Abstract】 Aim To investigate the effect of testosterone on intimal proliferation after rabbit abdominal aorta balloon injury. Methods 24 male New Zealand white rabbits were divided randomly into control group (n=8, sham castration), hypotestosteronemia group (n=8, castration) and testosterone replacement group (n=8, castration +testosterone undecanoate, 14 mg/kg). Abdominal aorta was not injured with 3 mm PTCA balloon until testosterone undecanoate had been injected for three days. After injury two weeks later, blood samples were obtained for detection of plasma testosterone, nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MDA), and all the abdominal aortas were excised to be analyzed by computer. Results The intimal area and PCNA proliferation index of control group and testosterone replacement group were significantly decreased than those of hypotestosteronemia group (P<0.01), together with a significant increase in NO plasma level and significant decrease in MDA plasma level. No difference was observed between control group and testosterone replacement group in all parameters. Conclusions Testosterone, at physiological level, inhibits the intimal proliferation induced by balloon injury in male rabbit abdominal aorta. It might be related to increasing the synthesis of NO and the effect antioxidation.更多还原