【摘要】 本文对老年 2型糖尿病患者血清脂蛋白 (a)水平及载脂蛋白 (a)多态性进行了分析 ,以探讨老年糖尿病与高脂蛋白 (a)血症的关系及其载脂蛋白 (a)遗传表型的分布特点 ,同时分析脂蛋白 (a)与糖尿病并发症的相关性。用酶联免疫吸附法测定脂蛋白 (a)水平 ,用十二烷基磺酸钠 -聚丙烯酰胺凝胶电泳和Westernblot技术测定脂蛋白(a)遗传表型。结果发现 :① 3组 (老年 2型糖尿病、非老年 2型糖尿病与老年健康对照组 )脂蛋白 (a)浓度差异无统计学意义 (P >0 .0 5 ) ,脂蛋白 (a)与血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇及其亚型、低密度脂蛋白胆固醇、载脂蛋白A和B水平无相关性 (P >0 .1) ;②老年 2型糖尿病组合并高血压、糖尿病性视网膜病变和糖尿病肾病者的血清脂蛋白 (a)浓度明显高于无合并相应疾病者 (P <0 .0 5或P <0 .0 0 1) ;③ 3组共检出 13种载脂蛋白 (a)基因型 ,在老年 2型糖尿病合并症患者中 ,低分子质量的S1和S2基因型明显多于其它组及无合并症者更多还原

【Abstract】 Aim This report presents the studies of sera levels of lipoprotein (Lp)(a) and phenotypes of apolipoprotein (a) in type 2 diabetes mellitus. The aims of this study were to explore the relationship between aged patients with type 2 diabetes mellitus and sera levels of Lp (a) and the distributive characteristics of genetic phenotypes of apo (a). Moreover the report present the studies the relationship sera levels of Lp (a) and the complications of the aged type 2 diabetic patients with hypertension or retinopathy or nephropathy. Methods Enzyme linked immunosorbent assay (ELLSA) was used to measure sera levels of Lp (a). The phenotypes of apo (a) were determined by SDS polyacrylamide gel electrophoresis (SDS PAGE) and Western blot analysis. Results and Conclusion 1. There was no significant difference in serum Lp (a) concentration among the three groups (P>0.05,respectively). Serum Lp (a) concentration did not significantly correlate with the levels of serum TC,TG,LDLC,HDLC,apo A,apo B (P>0.1,respectively). 2. In the aged type 2 diabetic patients with hypertension or retinopathy or nephropathy, the level of serum Lp (a) concentration was higher than that in patients without these complications (P<0.05 and P<0.001). 3. A total of 7 different phenotypes of apo (a) were determined among the three groups. The aged type 2 diabetic patient with complications mostly had S1 and S2 phenotypes than other groups and the aged type 2 diabetics without the complications.更多还原