【摘要】 利用刺吸电位 (EPG)及其即时中断技术研究了棉蚜Aphisgossypii传播黄瓜花叶病毒(CMV)的机理 ,分析电势落差波 (pd)及其亚波形在棉蚜获得CMV的取食行为过程中的作用。结果表明 :棉蚜的获毒需要pd波的发生 ,它的获毒效率与穿刺病株细胞膜的次数呈正相关。证明了这种行为是一种细胞内行为。对植物细胞内穿刺产生的电位落差 (pd)波亚波形分析的结果显示 ,棉蚜的获毒发生在pd波的Ⅱ 3阶段 ,与棉蚜主动吸食植物细胞汁液的活动相关 ,这支持了蚜虫获毒的“吸入假说”。更多还原

【Abstract】 The simultaneous display technique of electrical penetration graph (EPG) was used to study the effects of the wave potential drop(pd) and its sub-phases in the acquisition of cucumber mosaic virus(CMV) by the cotton aphid Aphis gossypii from the infected melon plants. The presence of the intracellular punctures indicated by the wave pd in EPG records is the prerequisite of the acquisition of CMV by aphids (1pd vs 0 pd: 47 1% vs 0%, P<0 001). The transmission efficiency was positively correlated with the number of pd(4pd vs 1pd: 80% vs 47 1%, P=0 023; 5pd vs 1pd: 90 5% vs 47 1%, P=0 001). The analysis of sub-phase showed that acquisition occurred primarily during the sub-phase Ⅱ-3 of intracellular punctures. This result supports the ‘ingestion hypothesis’ for nonpersistent virus acquisition.更多还原