【摘要】 目的　通过氨茶碱与茜素的显色反应来测定氨茶碱的含量。方法　用 72 1型分光光度计 ,利用分光光度法测定氨茶碱的含量。结果　在乙醇水介质中 ,氨茶碱与茜素发生显色反应 ,产物的最大吸收波长为 5 44nm ,表观摩尔吸光系数为 1 12× 10 4 L/mol/cm ,相对标准偏差为 0 98% (n =6 ) ,药物浓度在 5～ 45 μg/ml范围内呈线性关系。结论　本方法操作简便 ,条件易于控制 ,灵敏度高 ,检测限度低 ,对药物的测定结果令人满意更多还原

【Abstract】 Objective Todeterminetheaminophyllinecontentthroughreactionbetween aminophylline and alizarin.Methods By spectropholometry method with 721 type Results The reaction between aminophylline and alizarin took place in ethanol-water.The apparent molar obsorptivity was 1.12×10 4L/mol/cm at 544nm.The Beer’s law was conformed to in the range of 5～45μg/ml of aminophylline.Conclusion The method is simple with easily-controlled reaction conditions and highy sensitivity.更多还原