【摘要】 目的 :研究人膀胱移行细胞癌 (TCC)T2 4 细胞系中CyclinD1、CDK4 、p16和PCNA的表达及其意义。方法 :用免疫组化方法检测T2 4 细胞系在细胞周期各时期CyclinD1、CDK4 、p16和PCNA的表达。结果 :在G1、S和G2 M期 ,Cy clinD1阳性细胞数分别为 2 8.4%、46 .8%和 6 4.7% ;CDK4 阳性细胞数分别为 41.2 %、5 3.7%和 6 3.7% ;PCNA阳性细胞数分别为 41.3%、5 9.9%和 71.1%。在G1、S、G2 M期间均有显著性差异 (P <0 .0 1)。各期细胞中均未检出p16阳性细胞。结论 :(1)在正常 (非肿 )细胞中CyclinD1和CDK4 的表达主要在G1期 ,然而 ,在T2 4 细胞系中的表达在S期继续增高并在G2 M期达高峰 ,在肿瘤细胞中CyclinD1和CDKM4这种异常的表达形式和p16蛋白缺失可能导致细胞周期进程的正常调控机制丧失。 (2 )PCNA的表达状态可作为判断膀胱TCC组织病理分级和恶性程度的一个重要参考指标更多还原

【Abstract】 Objective: To study the expression of cyclin D 1?CDK 4?p16和PCNA and their significances in the human bladder transitional cell line T 24 .Expressions of cyclin D 1?CDK 4?p16 and PCNA at each phase of cell cycle in T 24 cell line were detected by immunohistochemistry. Results: at the G 1, S and G 2 M phase, positive cells of cyclin D1 were 28.4%, 46.8% and 64.7%; CDK 4 41.2%, 53.7% and 63.7%; PCNA 41.3%, 59.9% and 71.1%, resprectelly, and their differances between each phase were remarkable( P <0.001). The positive cell of p16 was undetected at the each phase of cell cycle in T 24 cell line. Conclusion: (1)Cyclin D 1 and CDK 4 belong to the G 1 classe of cyclins and CDKs but yet their expressions increased continually at S phase and peaked at G 2 M phase of cell cycle in T 24 cell line. These abnormal expressions of cyclin D 1 and CDK 4 and the deletion of p16 protein may lead to a loss of the normal regulator mechanisms of cell cycle progression in tumor cell.(2)The condition of PCNA expression can be regarded as a important parameter for evaluating histopathological grade and extent of malignancy of the bladder TCC.更多还原