【摘要】 借助超声波萃取法、以反相高效液相色谱 (RP -HPLC -DAD)为分析检测手段 ,研究了丹参 6种抗氧化成分的提取方法和分离定量条件 ,并对这些成分在全株丹参不同部位中的含量分布特征进行了探讨。结果表明 :常温下的超声波萃取可避免热解作用给丹参酮类化合物带来的损失 ,而辅助的加热处理可使原儿茶醛获得较高的提取率 ,两种操作方法简单可行。 6种化学成分在丹参不同部位及不同等级中的分布有较大差异。丹参酮类化合物主要分布在根部皮层 ,而叶子中丹参素的含量却较为丰富 ,可为丹参品质鉴定和质量标准研究及该资源的综合利用奠定理论基础。更多还原

【Abstract】 Extract method and determination parameters for 6 anti-oxidants from Salvia miliorrhiza Beg (Danshen) were reported by means of microwave oven and Reversed-Phase-High-Performance-Liquid-Chromatography (RP-HPLC). These components in different tissue of Danshen were also discussed. The results showed that the loss of tanshinones caused by heating depression could be avoided by means of microwave method at the room temperature, and a higher extraction yield was also obtained by heating. The two extraction methods were simple and easy to operate. The content of tanshinones and salvia acids varied with different tissue and grades. Tanshinones were mainly in cortex of Danshen root, and Danshensu in its leaves. It has great significance for Danshen identification and quality control.更多还原