【摘要】 目的 探讨ATP与COA活化癌性胸水肿瘤浸润淋巴细胞（TIL）的增殖力与杀伤活性。 方法 应用ATP和COA作为癌性胸水TIL的培养液，在7天、14天、21天、28天分别计算其增殖力，测定其杀伤细胞活性。 结果 单一的完全培养液TIL增殖量仅为（51．37±18．56）×108／ml，加入ATP和COA的完全培养液在14天时TIL增殖量即可达（105．31±24．76）×108／ml，扩增能力增加一倍以上。加入ATP和COA所培养的TIL其杀伤细胞活性明显增强，在14天百分比达到58．12±12．35, 21天百分比时可达78．50±11．93。同时可提前一周达到临床治疗胸腔积液输注所需的TIL数量。 结论 培养液中加入ATP和COA可以活化癌性胸水ITL的增殖力与杀伤活性，有一定的临床实用价值。更多还原

【Abstract】 Objective To study the proliferative ability and cytoxicity of tumor infiltration lymphocytesTILsactivated by ATP and COA from malignant pleural effusion． Methods TILs from pleural effusion were cultured in the medium conditioned with ATP and COA． The proliferation profile and cytoxicity were datemined on the 7th、14th、21st and 28th day respectively． Result The proliferated amount of TILs cultured in single complete medium was51．37±18．56×108／ml,while cultured by adding ATP and COA into the medium was（105．31±24．76）×108／ml after 14 days． Proliferative ability was more than doubled and had greater cytotoxicity against tumor cells．The cytotoxicity reached 58．12±12．35 on the 14th day and 78．50±11．93 on the 21st day．The requisite quantity for clinical therapy of pleural effusion could be achieved one week in advance． Conclusion Adding ATP and COA into the culture medium can activate proliferation and improve cytotoxicity of TILs． It is of certain value in clinical application．更多还原