【摘要】 为研究禾谷镰孢菌Fusarium graminearum Schw.单端孢霉烯族毒素生物合成基因（产毒基因）在寄主体内的表达，作者构建了带报告基因GUS（(-葡糖苷酸酶基因）的质粒pGUSTRI6P5，并通过对野生型菌株的转化获得禾谷镰孢高产毒菌株。该质粒含有由TRI5（禾谷镰孢单端孢霉二烯合酶基因）启动子（TRI5 Prom）驱动的GUS基因编码区、潮霉素B抗性基因和拟枝孢镰孢F. sporotrichioides的产毒调控基因TRI6（FSTRI6）。用pGUSTRI6P5转化野生型菌株GZ3639后，在含潮霉素 B的培养基上选取抗性菌落，单孢分离获单孢菌株（转化子）。在GYEP（葡萄糖-酵母粉-蛋白胨）液体培养基上，转化子B4-1和B16-1的GUS比活力强，15-AcDON（15-乙酰脱氧雪腐镰刀菌烯醇）产量高，且两者呈正相关（相关系数（r）分别为0.9839和0.9523）。B4-1和B16-1两个转化子可作为研究禾谷镰孢与其寄主相互作用的工具菌株。更多还原

【Abstract】 A wild type strain (GZ3639) of Fusarium graminearum Schw. was transformed using the plasmid pGUSTRI6P5, which was constructed in this study. This plasmid consists of the promoter of TRI5 (a trichothecene biosynthetic gene), followed by a coding region of the GUS reporter gene, a copy of TRI6 (a trichothecene biosynthesis regulatory gene from F. sporotrichioides), and a hygromycin B resistance gene. Following fungal transformation, hygromycin B resistant colonies (transformants) were selected and single-spored. Transformants B4-1 and B16-1 were analyzed for GUS specific activities and 15-AcDON (15-acetyldeoxynivalenol, a species of trichothecenes) production in liquid medium. Seven days after inoculation, the selected transformants produced about 1,200 (g/ml of 15-AcDON, a 240 fold increase compared to the production by the wild type GZ3639, while both transformants had high GUS specific activities. Therefore, there was a positive correlation between GUS specific activities and 15-AcDON production. Thus, B4-1 and B16-1 are useful tools for study on interaction between F. graminearum and its host wheat in relation of trichothecenes.更多还原