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应用5.8S rDNA及ITS区序列分析链格孢种级分类
APPLICATION OF SEQUENCING OF 5.8S rDNA, ITS1 AND ITS2 ON IDENTIFICATION AND CLASSIFICATION OF ALTERNARIA AT SEPECIES LEVEL
【摘要】 对9个链格孢小孢子种和3个大孢子种共20个链格孢菌株的5.8S rDNA及其两侧的ITS1区和ITS2区进行了序列分析。聚类分析结果表明形态差异大的种可以明确加以区分，而供试的9个小孢子种之间差异很小，不能根据对所选区段的序列分析加以区分。传统分类上的滨菊链格孢不属于Alternaria, 其分类地位需进一步研究。
【Abstract】 ： The sequences coding for the 5.8S rDNA and the flanking internal transcribed spacers (ITS1 and ITS2) were amplified by PCR and sequenced from 9 small-spored species and other 3 large-species in Alternaria. Phylogenetic analysis clearly separated the 2 large-spored species from each other and from 9 small-spored species tested, but small-spore species cannot be clearly separated each other. This indicate that small-spored species tested belong to a natural species group. Traditional taxonomic morphologically distinguished species A.leucanthemi may not belong to Alternaria according to sequence analysis, its taxonomic position require further study.
【Key words】 Alternaria； sequence analysis； 5.8S rDNA； ITS spacers；
- 【文献出处】 菌物系统 ,Mycosystema , 编辑部邮箱 ,2001年02期
- 【分类号】Q949.32
- 【被引频次】168
- 【下载频次】699