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表型相似的热带假丝酵母和麦芽糖假丝酵母在脉冲电泳核型上的差异(英文)

DIFFERENTIATION BETWEEN THE TWO PHENOTYPICALLY SIMILAR YEAST SPECIES CANDIDA MALTOSA AND CANDIDA TROPICALIS BY ELECTROPHORETIC KARYOTYPING

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【作者】 白逢彦梁慧燕贾建华

【Author】 BAI Feng-Yan, LIANG Hui-Yan, JIA Jian-Hua (Systematic Mycology & Lichenology LaboratoryInstitute of Microbiology, Academia Sinica, Beliing 100080)

【机构】 中国科学院微生物研究所真菌地衣系统学开放研究实验室!北京 100080

【摘要】 热带假丝酵母Candida tropicalis (Castellani )Berkhout和麦芽糖假丝酵母C. maltosaKomagata, Nakase & Katsuya是两种可利用烃类作为碳和能量来源的酵母菌,前者还是一种条件致病菌,可引起系统感染。这两种假丝酵母菌在形态和生理生化性状上非常相似,用常规分类方法不易准确地鉴别。本研究对C. tropicalis和C maltosa的模式菌株以及中国普通微生物菌种保藏中心(CGMCC)保藏的归于这两个种名下的其它菌株进行了脉冲电泳核型比较分析。发现这两个表型相似的种具有明显不同的染色体DNA分子带型,而同一种内的不同菌株却具有相同或相似的分子核型。C.tropicalis的特异染色体DNA分子带谱为2条8.5—1.2 Mb的带, 4条2.3-3.4 Mb的带。 C maltosa的特异带谱为: 3~4条分子量在1.1-1.3Mb范围内的带, 1条约为2.2Mb的带以及2-3条大小为3.2-3.5Mb的带。 C tropicalis与C maltosa在染色体DNA分子带型上的差异与二者在可溶性淀粉的同化能力和40℃下的生长能力上的差异具有明显的相关性,前者可同化可溶性

【Abstract】 The two hydrocarbon-utilizing yeast species Candida maltosa Komagata, Nakase & Katsuya and Candida tropicalis (Castellani) Berkhout are not easy to be clearly distinguished from each other by conventional methods because of their similar morphological, physiological and biochemical characters. Electrophoretic karyotypes of the strains preserved in the China General Microbiological Culture Collection Center (CGMCC) under these two species were analyzed by using pulsed field gel electrophoresis (PFGE). The type and representative strains of C. maltosa were clearly differentiated from those of C. tropicalis in chromosomal DNA banding patterns, while all the strains studied within each of these two species showed similar or identical electrophoretic karyotypes. The difference in electrophoretic karyotypes between C maltosa and C tropiclais coincided well with their different abilities to assimilate soluble starch and to grow at 40 C.

【基金】 国家自然科学基金资助项目 No. 39700001&&
  • 【分类号】Q939.5
  • 【被引频次】13
  • 【下载频次】134
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