【摘要】 目的 :克隆和鉴定IL 6作用相关基因将有助于揭示IL 6信号转导机制和发现新的信号分子。方法 :以来源于IL 6处理和未处理的U937细胞mRNA的双链cDNA作为检测子和驱动子 ,进行cDNA代表性差异分析。结果 :分离了 14个差异表达基因序列 (EST) ;反向RNANorthern杂交筛选到 9个差异表达序列 ,经测序及序列分析 ,其中 7个差异表达的EST代表在细胞生命活动或信号转导中有重要作用的已知基因 ,2个EST代表与IL 6作用相关的新基因。Northern印迹和时间表达谱进一步证明了P52和P31代表的新基因与IL 6作用的相关性。结论 :上述结果提示这些差异cDNA序列可能与IL 6信号转导作用密切相关更多还原

【Abstract】 Objective:To clone and identify the genes related to IL 6 in order to reveal mechanisms of IL 6 signal transduction and to discover new signal transduction molecules. Methods: The cDNA RDA method was utilized to both detect and isolate cDNA fragments from differentially expressed genes(EST) from the IL 6 induced and uninduced U937 cells. Results: A total of 14 differential ESTs were obtained, And then, 9 ESTs were proved to be IL 6 effect related genes by reverse Northern hybridization. Sequencing and bioinformatics analysis indicated that 7 gene fragments were known genes related with the cell life and signal transduction and 2 fragments were novel genes potentially related with IL 6. Further two novel genes (P52 and P31) were confirmed to be IL 6 effect related genes by Northern blotting. The time expression pattern analysis confirmed further that P31 and P52 represent the new genes related to IL 6 action. Conclusions: These results suggested that these cDNA sequences might be involved in IL 6 signaling. [更多还原