【摘要】 目的 :探讨本芴醇的抗疟作用机制。方法 :采用流式细胞术 (FCM)分析了本芴醇和氯喹对鼠伯氏疟原虫K173株DNA含量及溶酶体pH值的影响。结果 :对照组疟原虫DNA含量在各时间点没有显著变化。本芴醇单次给药后 ,随时间推移疟原虫DNA含量逐渐减少 ,药后 2h两给药组疟原虫红内期DNA含量开始降低 ,到 16h降到最低 ,但药后 2 4hDNA含量又有所回升。本芴醇单次给药后 1h起疟原虫溶酶体pH值开始升高 ,药后 3h升至最高 ,药后 4h原虫溶酶体pH值恢复至药前水平。对照药氯喹对疟原虫DNA含量和溶酶体pH值也有相同影响。结论 :本芴醇的抗疟作用与其抑制DNA合成相关 ,但与其升高溶酶体pH值的关系不明确更多还原

【Abstract】 Objective:To study the antimalarial mechanism of benflumetol (B). Methods: Flow cytometry (FCM) was used to analyze the effects of B and chloroquine (CQ) on DNA content of Plasmodium berghei and pH value of the lysosome of malarial parasites. Results: DNA content of the plasmodia not treated with any drugs was not changed in 24 hours,while benflumetol could decrease the DNA content: the DNA content began to decrease 2 h after the drug administration and reached the minimum by 16 h, but somewhat increased at 24 h after administration. The pH in the lysosome increased 1 h and restored premedication level 4 h after benflumetol administration. Chloroquine had the same effects on DNA and lysosome pH of malarial parasites.Conclusions: The antimalarial mechanism of benflumetol is directly related to its effect to inhibit the synthesis of DNA. [更多还原