【摘要】 目的　通过检测Bcl 2蛋白在正常各期胎盘、妊高征胎盘和胎儿宫内发育迟缓胎盘中的表达 ,探讨Bcl 2蛋白在胎盘的发育过程中的功能及妊高征和胎儿宫内发育迟缓的病理机制。　方法　取正常的 8～ 10周、2 0～ 2 2周、足月胎盘以及妊高征和胎儿宫内发育迟缓的胎盘组织固定 ,石蜡包埋 ,用ABC法抗Bcl 2免疫组织化学染色 ,光镜观察。　结果　正常早孕绒毛合体细胞滋养层、细胞滋养层细胞核、绒毛中轴细胞阳性 ,细胞滋养层的胞质、绒毛中轴基质阴性。正常中期和足月胎盘滋养层细胞、血管内皮细胞阳性。与正常足月胎盘相比 ,妊高征、胎儿宫内发育迟缓胎盘几乎不着色。　结论　Bcl 2蛋白在正常各期胎盘中均有表达 ,而在妊高征和胎儿宫内发育迟缓胎盘几乎不表达 ,Bcl 2蛋白表达异常与妊高征与胎儿宫内发育迟缓的发病机制有关更多还原

【Abstract】 Objective To detect the expression of Bcl\|2 protein in normal, preeclampsia, intra\|uterine growth restriction(IUGR) placenta so as to study its function during differentiation of trophoblasts and the molecular mechanism of preeclampsia and IUGR. Methods The normal placental tissues(8\|10?week,20\|22?week and 39\|41?week),preeclampsia placental tissues, intra\|uterine growth restriction(IUGR) placental tissues were fixed, embedded,sectioned,immunohistochemically stained with monoclonal anti\|Bcl\|2(ABC method) and observed under the microscope. Results In the early pregnant chorion, the syncytotrophoblasts(ST), the nuclei of cytotrophoblasts(CT) and the cells in the core of first trimester were Bcl\|2 protein positive.The plasma of CT and the matrix of the core of first trimester were Bcl\|2 protein negative.The trophoblasts and endotelium of the third trimester of mid\|term and term placentae were positive. By contrasting, preeclampsia placental tissues and IUGR placental tissues were nearly unstained. Conclusion Bcl\|2 protein was expressed in normal placenta of different period and was rarely expressed in preeclampsia placental tissues and IUGR placental tissues. Decreased expression of Bcl\|2 might be related to mechanism of preeclampsia and IUGR.更多还原