【摘要】 目的　为了进一步研究缝隙连接 (gapjunction ,GJ)在生长控制中的作用 ;探讨抑制GJ形成的可能机制。方法　把一种专一于鸡眼晶状体纤维细胞质膜中的MIP(majorintrinsicprotein) ,称为ND6的单克隆抗体注入 2 0期鸡胚右眼内 ;左眼不注射 ,作为对照。ND6处理 2 4h后分析晶状体 ,包括完整晶状体和匀浆晶状体的蛋白磷酸化水平变化。结果　处理眼的晶状体明显大于对照眼的晶状体 (长径P <0 0 1;宽径P <0 0 0 1) ;处理晶状体的蛋白磷酸化水平增高 (与对照晶状体比较 ,完整晶状体增高 76 % ;匀浆晶状体增高 2 0 5 % )。结论　ND6能引起晶状体的过度生长 ;这种晶状体的不正常生长可能是因为GJ的形成被抑制而引起。MIP是本实验中被磷酸化的蛋白 ,它的磷酸化可抑制GJ的形成更多还原

【Abstract】 Objective In order to further study the role of gap junction(GJ) in growth control; and explore the possible mechanisms for the inhibition of GJ formation. Method A monoclonal antibody,called ND6, which is specific for MIP(major intrinsic protein)in the plasma membrane of eye lens fiber cells in chicken,was injected into the right eyes of chicken embryos at development stage 20, while the left eyes were not injected and served as controls. The size of the lenses was measured 24 hours after the ND6 treatment; the protein phosphorylation of the lenses, including intact and homogenized lenses was also analyzed 24 hours after the ND6 treatment. Results The size of the lenses from treated eyes was significantly larger than those of the control ones( P <0 01 in length and P <0\^001 in width); and the levels of protein phosphorylation from the treated lenses were also increased (76% higher in the intact lenses and 205% higher in the homogenized lenses, when compared to the control lenses). Conclusion The results in this study suggest that ND6 can induce overgrowth in lenses, and that abnormal growth in lenses can be caused by inhibition of GJ formation. It is proposed that MIP might be the protein whose phosphorylation was also affected in this study, its phosphorylation in turn may lead to the inhibition of GJ formation. [更多还原