【摘要】 目的　研究被LPS和IFN γ激活的巨噬细胞的肌动蛋白分布和钙离子水平 ,探讨肌动蛋白构成和钙离子浓度在巨噬细胞游走和吞噬方面的作用。　方法　LPS和IFN γ激活大鼠巨噬细胞后 ,用phallacidin和Fura 2标记肌动蛋白和游离钙离子 ,共聚焦激光扫描显微镜和钙离子图像分析仪下观察肌动蛋白分布和分析钙离子水平。　结果　在被激活的巨噬细胞 ,游走细胞和吞噬细胞的数量增加。细胞内F 肌动蛋白增多 ,钙离子水平升高。游走细胞有头部和尾部 ,头部伸出板状伪足和丝状伪足。板状伪足和丝状伪足内含有丰富的F 肌动蛋白。细胞底部出现肌动蛋白丝构成的应力纤维。在游走细胞 ,尾部的钙离子水平比头部高。游走细胞转向时 ,弯曲部的钙离子水平明显升高 ,特别是弯曲部的外侧区。巨噬细胞吞噬淋巴细胞时 ,吞噬处的钙离子水平显著升高。　结论　巨噬细胞被LPS和IFN γ激活时 ,游走和吞噬功能明显增强 ,细胞内F 肌动蛋白分布和钙离子水平发生了特征性的形态学变化更多还原

【Abstract】 Objective To investigate the distrbution of actin and the level of Ca 2+ in macrophages activated with LPS and IFN\|γ and study effects of reorganization of actin and the level of Ca 2+ on migration and phagocytosis of macrophages. Methods Rat macrophages were activated with LPS and INF\|γ,actin and intracellular free Ca 2+ were labeled with phallacidin and Fura\|2,the distribution of actin and the level of Ca 2+ were observed and analyzed with Ca 2+ image analysis system and confocal laser scanning microscope. Results The migrating cell and the phagocytizing cells increased in the activated macrophages.F\|actin and the level of Ca 2+ of the activated macrophages increased.There was a front and a back in the migrating cell.Lamellipodia and filopodia protruded from the front.F\|actin of the lamellipodium and filopodium was rich.There were stress fibers of F\|actin at the bottom of the migrating cells.In the migrating macrophages,the level fo Ca 2+ in the back was high than that in the front.When the migrating cell turned their growth direction,the level of Ca 2+ at the curve increased greatly,especially in its lateral region.When macrophage phagocytized the dead lymphocyte,Ca 2+ in the phagocytizing region increased. Conclusion When macrophages are activated with LPS and IFN\|γ,migration and phagocytosis increase greatly.Particular morphologic changes in the distribution of F\|actin and the level of intracellular free Ca 2+ occur. [更多还原