【摘要】 目的　通过阻断 T细胞的 Fas信号传递途径 ,探讨消除肿瘤对 T细胞的攻击及其对肿瘤的治疗意义。　方法　流式细胞术、RT- PCR方法检测卵巢癌细胞表达 Fas和 Fas L。构建 pc DNA3-反义 Fas真核表达载体 ,经脂质体转染 Jurkat细胞 ,流式细胞仪检测 Fas表达变化。以 Annexin- V和 MTT法检测转染反义 Fas基因对 Ju-rkat细胞凋亡的影响。采用 MTT体外杀伤实验观察 3AO对 Jurkat细胞杀伤变化。　结果　 6种卵巢癌细胞均表达Fas和 Fas L。pc DNA3-反义 Fas基因可以使 Jurkat细胞表达 Fas量下降并部分阻断 Fas单抗诱导的 Jurkat细胞凋亡 ,3AO对其杀伤减弱。　结论　卵巢癌细胞表达 Fas L可能是其逃逸免疫监视并产生对淋巴细胞攻击的原因之一 ;应用反义技术阻断 Fas表达 ,可部分阻断 Fas单抗诱导 Jurkat细胞凋亡 ,可削弱肿瘤细胞对淋巴细胞的攻击。更多还原

【Abstract】 Objective\ By inhibiting approach of apoptosis of T cells to observe if it could reduce the attacks of tumor cells upon lymphocytes and its possible role in tumor treatment. Methods\ The expressions of Fas/FasL genes in ovarian carcinoma cells were detected by using flow cytometry and RT\|PCR method.The construction of pcDNA3\|antisense Fas,the constructed vector was transfected into Jurkat cells with lipofectin,the change in expression of Fas gene was determined by flow cytometry.By means of Annexin\|V and MTT the effect of apoptosis in transduced Jurkat cells was investigated,and also using MTT cytotoxic test to investigate how 3AO cells kill Jurkat cells. Results\ Fas/FasL were expressed in 6 ovarian carcinomal cells.The expression level of Fas protein in Jurkat cells transduced with the constructed vector was decreased.Apoptosis was reduced in antisense Fas\|transfected Jurkat cells after anti\|Fas treatment. Conclusion\ FasL expression in ovarian carcinoma may be one of the reasons for ovarian carcinoma to escape immunosurveillance and attacking lymphocytes.Blocking Fas expression in lymphocytes can partially inhibit Jurkat cells apoptosis induced by anti\|Fas and reducing the attacks of tumor cells upon lymphocyte.\;更多还原