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光密度法测定微藻生物量

An optical density method for determination of microalgal biomass

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【作者】 沈萍萍; 王朝晖; 齐雨藻; 谢隆处; 王艳;

【Author】 SHEN Ping-ping,\ WANG Zhao-hui,\ QI Yu-zao, \ XIE Long-chu, \ WANG Yan (Institute of Hydrobiology, Jinan University, Guangzhou 510632, China) [

【机构】 暨南大学水生生物研究所!广东广州510632;

【摘要】 目的 :为准确而又快速的测量微藻生物量 .方法 :选用 15种不同微藻 ,在实验室中分别测定其细胞密度及光密度并进行直线回归分析 ,同时采用吸光系数来估算浮游植物生物量 .结果 :得出了浮游植物吸光系数与细胞碳含量 (即生物量 )的回归方程 :lg(m) =- 1.0 4 65ln(x) +4.2 551.结论 :这是一种利用光密度法来测量微藻生物量的简单有效的方法

【Abstract】 Aim: To investigate the relationship between optical density (OD) and the biomass of microalgae. Methods: 15 microalgal species were used under laboratory conditions to study the relationship between the cell density and its related OD. Meanwhile, the optical absorption coefficient, namely the slope of the linear graph which showed the relationship between cell density and OD, was utilized to estimate the phytoplankton biomass. Results: Regression analysis results showed the linear function: lg( m )=-0.451 1 ln( x )+4.242 2, in which c is the carbon content and x is the optical absorption coefficient of microalgae. Conclusion: This is a useful and rapid OD method to measure the biomass of microalgae . [

  • 【文献出处】 暨南大学学报(自然科学与医学版) ,Journal of Jinan University(Natural Science & Medicine Edition) , 编辑部邮箱 ,2001年03期
  • 【分类号】Q949
  • 【被引频次】205
  • 【下载频次】2565
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