【摘要】 目的 :了解逆转录病毒介导的 Hy TK基因转移联合更昔洛韦 (GCV )对肾癌细胞的杀伤作用。　方法 :利用逆转录病毒介导将 Hy TK基因导入人肾癌细胞株 GRC- 1,经用潮霉素筛选 ,获得稳定表达 Hy TK基因的 GRC- 1/Hy TK细胞 ;应用 PCR、RNA、Dot blot分子杂交及 MTT等方法检测 ,在 DNA、RNA水平的表达以及 Hy TK联合GCV对 GRC- 1/ Hy TK细胞的杀伤作用。　结果 :PCR、Dot blot检测证明 ,Hy TK基因的整合及 m RNA水平的表达 ;MTT法测定示 GCV对 GRC- 1/ Hy TK细胞有明显的杀伤作用。其 IC5 0 值为 3.49μg/ m l,而对其亲代 GRC- 1细胞无明显杀伤作用。　结论 :逆转录病毒介导的 Hy TK基因转移联合 GCV可作为肾癌基因治疗的一种有效方法。更多还原

【Abstract】 Objectives:To evaluate the therapeutic efficacy of retroviral mediated hygromycin phosphotransferase thymidine kinase fusion gene(HyTK)/GCV on human renal carcinoma cell. Methods:A HyTK transferred renal carcinoma cell line has been established in vitro by retroviral mediated gene transfection technique. The HyTK gene transferred cell line were measure by PCR, Dot blot and antitumor effects were observed after GCV treatment. Results: In vitro experiments demonstrated that GRC 1 cells transferred by HyTK gene were killed after GCV treatment. Conclusions:Retroviral mediated HyTK/GCV system was a promising suicide gene therapy for renal carcinoma.更多还原