【摘要】 目的　研究葛根素 (Puerarin ,Pue)对氧自由基的清除以及抗氧化性损伤作用。方法　以核黄素 -光系统产生超氧阴离子 ,Fenton反应产生羟自由基 (·OH) ,研究Pue对氧自由基的清除作用 ;以过氧化氢 (H2 O2 )引起红细胞溶血 ,黄嘌呤 -黄嘌呤氧化酶 (X -XOD)致豚鼠心室乳头肌损伤 ,研究Pue的抗氧化性损伤作用。结果　Pue能清除超氧阴离子和·OH ;抑制H2 O2 引起红细胞溶血和脂质过氧化物生成 ;增强心室乳头肌超氧化物歧化酶 (SOD)活性 ,降低丙二醛 (MDA)含量 ,保护乳头肌免受超氧阴离子的损伤。结论　Pue对氧自由基有清除作用 ,并能预防性对抗H2 O2 和超氧阴离子引起的氧化性损伤更多还原

【Abstract】 Aim To study the effects of Puerarin (Pue) on the scavenge of oxygen free radicals (OFR) and the antagonism against oxidative injury.Methods The effects of Pue on the scavenging activity on OFR including superoxide anion and hydroxgl radical (·OH),the inhibitory effects on the oxidative hemolysis induced by hydrogen peroxide (H 2O 2) and the protective effects against ventricular papillary muscles injury induced by xanthine-xanthine oxidase (X-XOD) were discussed in this paper.Results Pue could inhibit superoxide anion generated by riboflavin system and ·OH generation according to Fenton reaction.The oxidative hemolysis and the produce of lipid peroxidation of erythrocytes induced by H 2O 2 were also inhibited by Pue.Furthermore,it also inhibited the decrease of the activity of superoxide dismutase (SOD) and the increase of the content of malonadehide bis (MDA) and thus prevented ventricular papillary muscles from being harmed by superoxide anion.Conclusion It is indicated that Pue is the scavenger of OFR and is capable of prophylacticly antagonizing against the oxidative injury by H 2O 2 and superoxide anion.更多还原