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糖尿病大鼠血管糖基化终产物含量与其受体和ICAM-1表达的关系

Relationship between the advanced glycation end products content and expressions of RAGE,ICAM-1 in vascular tissue of diabetic rats

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【作者】 张建伟孙仁宇

【Author】 ZHANG Jian wei, SUN Ren yu (Institute of Basic Medical Sciences, PUMC & CAMS, Beijing 100005,China)

【机构】 中国医学科学院中国协和医科大学基础医学研究所!北京100005

【摘要】 探讨糖尿病大鼠血管组织糖基化终产物 (AGEs)含量与其受体 (RAGE)和细胞间粘附因子 1(ICAM 1)表达的关系。复制糖尿病大鼠模型 ,采用荧光法、RT PCR及原位杂交方法检测主动脉及心肌组织的AGEs含量以及RAGE和ICAM 1基因的表达。发现糖尿病大鼠主动脉和心肌组织AGEs含量升高 (P <0 0 1) ;RAGE和ICAM 1基因表达增强 (P <0 0 5~ 0 0 1) ;AGEs含量与RAGE及ICAM 1呈明显正相关 (P <0 0 1) ;氨基胍治疗可缓解上述指标的变化。提示AGEs可诱导RAGE和ICAM 1的表达。推测AGEs RAGE相互作用是引起糖尿病血管内皮细胞功能紊乱和损伤的关键环节。

【Abstract】 In this study, the relationship between the advanced glycation end products(AGEs) and the expressions of receptor for AGEs(RAGE), intercellular cell adhesion molecule 1(ICAM 1) was investigated The diabetic rat model was reconstructed and the fluorescence method, RT PCR and in situ hybridization techniques were used to detect AGEs content and the expressions of RAGE and ICAM 1 gene in the aorta and cardiac tissues The results showed that AGEs content in aortic and cardiac tissues increased( P <0 01) in diabetic rats; The expressions of RAGE and ICAM 1 enhanced ( P <0 05~0 01) and were positively correlated with the quantity of AGEs accumulation( P <0 01) in the aorta and cardiac tissue These parameters change in the diabetic rats can be improved with aminoglumine(AG) treatment Suggesting that AGEs might induce RAGE and ICAM 1 expression It’s postulated that AGEs binding to RAGE play an important role to result in diabetic endothelial cells dysfunction and lesion

  • 【文献出处】 基础医学与临床 ,Basic Medical Sciences and Clinics , 编辑部邮箱 ,2001年02期
  • 【分类号】R587.1
  • 【被引频次】15
  • 【下载频次】180
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