【摘要】 目的　观察乙型肝炎基因重组疫苗的安全性和对大学生的免疫效果 ,为制定免疫策略提供依据。方法　应用乙肝酵母重组疫苗 ,按 0 ,1和 2月 3针接种乙肝血清标志物全阴的 15 6名大学生 ,并用定量放免法(PIA)对免疫后的抗体反应进行检测。结果　未发现接种对象出现严重副反应。完成全程免疫后 2个月时 ,抗体阳转率达 81 41% ,但大多数接种者抗体滴度 (meangeometrictiter ,GMT)在 (10～ 99)IU/L之间 ,为低应答水平 ,只有 1 92 %的人GMT在 10 0IU/L以上 ,男、女生之间抗体阳转率及GMT构成分布差异多无显著性。 12名既往疫苗接种抗体无应答者复种疫苗后 ,有 7人抗体转阳。结论　乙肝重组疫苗安全性较好 ,对大学生免疫后的抗体阳转率尚可 ,但抗体应答水平较低 ,性别差异不明显。免疫失败后可考虑复种 ,以增加抗体阳转机会。更多还原

【Abstract】 Objective To evaluate the safety and immunogenicity of recombinant hepatitis B(HB)vaccine to undergraduates and lay a basis for formulaing an immunization strategy.Method Yeast derived recombinant HB vaccine(YDV)was given at 0,1 and 2 months to 165 undergraduates whose hepatitis B virus markers (HBVM) were negative before vaccination,and then to detect the titer of antibody against hepatitis B surface antigen(anti HBs)with radioimmunoassay(RIA).Result No severe adverse reaction was found in all vaccinee.Positive seroconvertion rate was 81.41 percent two months after three dose schedule,but most subjects were lowresponders,whose mean geometric titer(GMT)were at 10 99IU/L.Only 1.92 percent of the subjects reached the level that is higher than 100IU/L.7 of 12 non responders to primary HB vaccination showed response to HB antigen after our 3 dose schedule.Conclusion Safety and immunogenicity of recombinant HB vaccine is good for undergraduates,but the levels of anti HBs are too low.This response seems not to be influenced by sex.Non responders to primary HB Vaccine should be given it again in order to reach sufficient anti HBs levels.更多还原