【摘要】 根据伪狂犬病病毒国外Ka株UL5 4基因核苷酸序列 ,设计一对包含UL5 4基因完整编码区的引物 ,以伪狂犬病病毒国内地方分离株Ea株细胞感染物为模板 ,PCR扩增出大小约 1.1kb特异性带。将纯化的扩增产物克隆到pBluescriptⅡsk +中 ,酶切分析证实后经双脱氧末端终止法进行全序列测定 ,序列分析结果表明Ea株UL5 4基因全长 10 86bp ,可编码 36 1个氨基酸。由二级结构预测数据推断C 末端具有典型的锌指结构域。运用Blast软件与Ka株UL5 4基因进行同源比较 ,发现Ea株UL5 4基因在核苷酸和氨基酸水平上均存在多处点突变 ,但无插入或缺失突变 ;将Ea株UL5 4氨基酸序列同其它 4种α 疱疹病毒 (HSV 1、HSV 2、VZV、EHV 1)进行同源比较 ,同源性分别为 47%、36 %、36 %和 44 %。更多还原

【Abstract】 About 1.1 kb DNA fragment encoding the early protein UL54 of pseudorabies virus (PRV) Ea strain was amplified by PCR technique and cloned into pBluescriptII sk+,resulting in the recombinant plasmid pSKBE. The nucleotide and deduced animo acids sequences indicated that the UL54 gene of PRV Ea strain was composed of 1086 base pairs and could encode 361 animo acids residues. The result of predicted II class structure showed that UL54 contained typical zinc finger motif within the C terminal. When compared with PRV Ka strain, there were multiple site mutations in the UL54 gene of PRV strain Ea, and the diversity of amino acid residues also existed. When compared with other alpha herpesviruses, such as HSV 1, HSV 2, VZV, and EHV 1, the homology values were 47%, 36%, 36% and 44% respectively.更多还原