【摘要】 通过采用以单片机为核心的数字电路控温技术、PWM技术以及PID控温算法 ,研制出成本低、控温精度高、能用于DNA扩增的PCR芯片温度控制系统更多还原

【Abstract】 A temperature controlled system of PCR chips for DNA amplification which possesses the characteristics of low cost and high controlling precision is developed by using digital circuit temperature controlled technique based on microcontroller, PWM technique and PID temperature controlled algorithm. The experimental results show that this system can well perform cycling control between different temperatures (37?℃,55?℃,72?℃and 94?℃)required for PCR chips and its controlling precision is ±0.1?℃ . The thermoelectric cooler is used as thermal generator and a heat sink (by controlling the polarity of supply power) and rise/fall rates of temperature are 4?℃/s and 2.2?℃/s respectively.更多还原