【摘要】 目的　分析PTEN基因在前列腺癌中的突变情况。方法　对 80例前列腺癌冰冻组织 ( 60例来自前列腺癌原发部位 ,2 0例来自盆腔淋巴结转移灶 )中PTEN基因进行聚合酶链反应 -单链构象多态性分析(PCR -SSCP) ,检测PTEN基因突变。结果　共有 32例发生PTEN基因突变 ,突变发生率为 4 0 % ( 32 / 80 )。来自前列腺癌原发部位的 60例标本中有 18例发生PTEN基因突变 ,突变发生率为 30 % ( 18/ 60 ) ;来自盆腔淋巴结转移灶的 2 0例标本中共有 14例发生PTEN基因突变 ,突变发生率为 70 % ( 14/ 2 0 )。后者PTEN基因突变发生率显著高于前者 ( χ2 =10 ,P <0 .0 0 5)。结论　PTEN基因突变在前列腺癌发生、发展、转移中起重要作用。PTEN基因可作为完善前列腺癌诊断的指标及前列腺癌联合基因治疗方案中的目的基因更多还原

【Abstract】 Objective:To analyze the mutation of the PTEN (phosphatase and tensin homology deleted on chromosome ten) gene in prostate cancer. Methods: The PTEN gene mutations are investigated by PCR SSCP (single strand conformation polymorphism analysis of polymerase chain reaction products)from 80 frozen prostate tumor specimens. Results:32 cases of PTEN gene mutations have been identified from 80 frozen prostate tumor specimens(the ratio is 40%).There are 18 cases have PTEN gene mutations out of 60 samples of clinically localized tumors (the ratio is 30%)and 14 cases have such mutations out of 20 samples of pelvic lymph node metastases(the ratio is 70%).The ratio of PTEN gene mutation in the latter is significantly different from that of the former(χ 2=10, P<0.005). Conclusion:the result of this study indicated that mutation in the PTEN gene is present mainly in metastates of prostate cancers,and may have an important effect on the generation and development and metastate of prostate cancer.更多还原