【摘要】 本文用噻唑盐比色方法探讨在 60 Co辐射损伤条件下扇贝多肽对胸腺细胞的保护作用 ,和对 60 Co辐射损伤的胸腺细胞修复能力的影响。结果表明 :(1) 60 Co辐射后胸腺细胞的活性明显下降。 (2 )先加扇贝多肽后辐射组的胸腺细胞的活性较未加扇贝多肽的辐射组强 (P<0 .0 5 ) ,0 .2 5 %～ 4 %浓度范围内 ,胸腺细胞的活性与扇贝多肽的浓度呈正比。当辐射强度加大到 9～ 10 GY时 ,0 .5 %以下的扇贝多肽保护能力减弱或丧失。(3)先 60 Co辐射后 2 h,再加入扇贝多肽孵育 ,胸腺细胞的活性随扇贝多肽浓度的加大而增强。辐射后 7h或 19h再加入扇贝多肽 ,仅在辐射 7h后加入 2 %的扇贝多肽孵育的胸腺细胞的活性较对照组活性强 ;辐射后 19h再加入扇贝多肽组的胸腺细胞的活性均较对照组显著降低 (P<0 .0 5 )。结果提示 :提示扇贝多肽具有抵抗 60 Co辐射对胸腺细胞的损伤作用 ,并且呈剂量依赖性 ;而且在一定的时间范围内对受到辐射损伤后的胸腺细胞具有促进其修复的作用更多还原

【Abstract】 In this paper the protective effect and facillitating reconverting effect of polypeptide from chlamys farreri (PCF) on thymocytes irradiated by 60 Co γ ray were studied using the MTT chromatometry .The results showed that:(1)the damaged extents of thymocytes were increased with the radiative intensity of 60 Co γ ray elevated at the range of 3GY to 9GY compared with that in no-exposure to 60 Co γ ray group.(2)PCF could reduce 60 Co γ ray damage on thymocytes with dose dependence .In the 0.25%~4% concentration range the higher concentration of PCF the stronger protective effects of it ,but the protective effect of PCF at the concentration below 0.5% disappeared while the radiative intensity of 60 Co γ ray was at 9GY.(3)PCF could facilitate the renovation ability of thymocytes after exposure 60 Co γ ray for two hours .And only 2% concentration of PCF showed the facilitating repair process of thymocytes after radiated by 60 Co γ ray for 7 hours .(4) PCF could decrease the activity and facilitate the death of the dying thymocytes after exposure 60 Co γ ray for 19 hours. The results suggested that the protective effect and facilitating repair effect of PCF on the thymocytes damaged by 60 Co γ ray may be mediated by the antisuperoxidation of PCF.更多还原