【摘要】 对真鲷精子超低温保存技术进行了初步探讨 ,在液氮中保存真鲷(Pagrosomusmajor)精子时发生冷冻伤害的温域为 -30～ -60℃ ,适宜的降温速率为20℃/min ;使用抗冻剂DM SO的适宜浓度为20% ;采用低盐度或高 pH值的溶液激活冻精的效果不如自然海水。真鲷冻精的复苏率和存活率最高可达到50.73%和62.5% ;以20℃/min和20%DMSO处理真鲷精子 ,保存在液氮中46d后冻精复苏率和存活率为50.63 %和61.02%。更多还原

【Abstract】 For sperm of Pagrosomus major, the freezing damage occurred at -20 ℃ to -60 ℃. The nature seawater is a kind suitable activator solution. The optimal motility and survival rate of 50.73% and 62.5% were obtained in cryopreservation of the spermatozoa of P.major using a cryoprotectants dilution (DMSO 20%+sucrose 5%) and freezing rate 20 ℃/min. The motility and survival rate of sperm is 50.63% and 61.02% respectively after storaging 66 days in LN 2.更多还原