【摘要】 对黑鲷精子的超低温保存技术进行了初步探讨 ,黑鲷(Sparusmacrocephalus)精子经过超低温保存后复苏率、存活率最高可以达到61.37 %和61.4 %。保存黑鲷精子时发生冷冻伤害的温度范围是 -20～ -60℃ ,适宜的降温速率为20℃/min ;使用抗冻剂DMSO的适宜浓度为20 % ;样品体积对保存效果有相关性 ;利用自然海水激活冻精效果好于低盐度或高 pH值的溶液 ;使用20 %DMSO和20℃/min降温速率处理 ,在液氮中保存黑鲷精子66d后解冻 ,其复苏率和存活率分别为59.81%和58.45%。更多还原

【Abstract】 In the present paper, attempting to develop simple,reliable and efficient methods for cryopreservation are reported. Cryopreservation of sperm cells of black porgy (Sparus macrocephalus) was investigated in liquid nitrogen. Motility and survival rate of 61.37% and 61.4% were obtained in cryopreservation of the spermatozoa of this species using a cryoprotectants dilution (DMSO 20%+sucrose 5%) and freezing rate 20 ℃/min. The volume of samples can affect the result. The nature seawater is better than lower osmotic or high pH activator solution. The freezing damage occurred at -20 ℃ to -60 ℃ for S. macrocephalus. The motility and survival rate of sperm is 50.63% and 61.02% after storaging 66 days in LN 2.更多还原