【摘要】 以灭活溶藻弧菌（０．０５％甲醛，３０℃，８ｈ）制成菌苗，注射免疫实验兔获得抗血清。该 抗血清经吸附后与副溶血弧菌、河流弧菌等１０株对照菌株无交叉反应。用该抗血清进行溶藻 弧菌ＥＬＩＳＡ检测，其最低检测极限为９７０００ｃｆｕ／ｍｌ。将该方法应用于养殖现场，检测结果为：海 水中溶藻弧菌浓度均低于最低检测限、外观健康大黄鱼肝脏中溶藻弧菌的阳性检出率为 ２０％、患病大黄鱼的阳性检出率为８０％，表明ＥＬＩＳＡ法不仅可以用于诊断大黄鱼的溶藻弧菌 病，也可以检测无病症带菌大黄鱼。更多还原

【Abstract】 Vibrio alginolyticus, the pathogenic bacteria of Pseudosciana crocea was inactivated by the addition of formaldehyde to 0. 05% (30 ℃, 8 h) for vaccine. Anti- V. alginolyticus serum was obtained from rabbits by injecting vaccine. The anti serum has no cross reaction to V.parahaemduticus and other nine strains after being absorbed. Anti serum wes used for V. alginolyticus detecting by ELISA method. The lowest V. alginolyticus suspension detectable was 97 OOO cfu/ ml. This ELISA method was used for pathogenic bacteria detecting in aquatic farming P.,crocea. The results showed that V. alginolyticus in seawater was undetectable; 80% liver of diseased P. crocea were positive in V. alginolyticus while in the normal, 20%. The results indicated that ELISA method could be used not only to diagnose the clinically diseased P. crocea, but also to recognize the carrier.更多还原