【摘要】 目的　建立人血清高密度脂蛋白磷脂 (HDL- PL)的测定方法。方法　采用磷钨酸及氯化镁沉淀法将血清中含载脂蛋白 B脂蛋白沉淀去除 ,上清液用于 HDL- PL 测定。结果　只需 2 .5～ 3.0分钟时间就能将HDL-磷脂完全消化。血清 HDL- PL 测定结果的变异系数为 3.6%～ 3.7% ,回收率为 98%～ 10 7% ,平均回收率为10 3%。对 30例正常人、30例 a型高脂蛋白血症、30例 型高脂蛋白血症及 30例 b型高脂蛋白血症患者血清HDL- PL 及 HDL- C进行了测定 ,发现 型高脂蛋白血症患者血清 HDL- PL 显著低于正常人和 a型高脂蛋白血症患者 ,其它各组之间血清 HDL- PL 无显著性差异。而 HDL- C在正常人及 a型高脂蛋白血症患者与 b型高脂血症患者及 型高脂血症患者间比较均有显著性差异。结论　人 HDL- PL 代谢与体内甘油三酯代谢的关系更为密切 ,HDL- PL 降低可能是检测 型高脂蛋白血症的更好的指标更多还原

【Abstract】 Objective To develop an assay for high density lipoprotein phospholipids in human serum based on ascorbutate reduction method. Methods HDLs were separated from apolipoprotein B containing lipoproteins by precipitation of phosphotungstic acid and magnesium chloride. Phospholipids of HDL were extracted by ethanol/ether, and dried. After the dried phospholipids were digested by sulphuric acid and perchloric acid, the color was developed by adding ammonium molybdate in ascorbutate. The levels of high density lipoprotein phospholipids (HDL PL) were measured by spectrophotometry at 700 nm. Results The coefficients of variation (CV) were 3.6% and 3.7% within two batches of assays. Recovery of isolated HDL PL added to serum ranged from 98% to 107%, averagely 103%. The established assay for human serum HDL PL was used to measure the serum levels of 30 hypercholesterolemic subjects, 30 hypertriglyceridemic subjects, 30 combined hyperlipidemic subjects, and 30 normolipidemic subjects. The hypertriglyceridemic subjects had lower HDL PL level than normolipidemic subjects and hypercholesterolemic subjects (The P values are 0.005 and 0.007 respectively). Conclusion A simple and specific method for assay of HDL phos pholipids in human serum has been developed. The above data collected by the use of this method demonstrate the closer relationship between human HDL PL metabolism and triglyceride metabolism, suggesting that lower HDL PL level might serve as an index in the assay for type Ⅳ hyperlipidemia.更多还原