【摘要】 目的　观察槲皮素 (Que)及异鼠李素 (Iso)对 Cu2 +介导的极低密度脂蛋白 (VL DL)氧化修饰的影响。方法　采用一次性密度梯度离心法分离人血浆 VL DL,用 Cu2 +进行体外氧化修饰 ,抗氧化组在温育前加入不同浓度的 Que和 Iso。分别检测脂蛋白中丙二醛 (MDA)、维生素 E(Vit E)及超氧化物歧化酶 (SOD)活性。结果　Que和 Iso可明显降低 OX- VL DL 中 MDA含量 ,延缓 OX- VL DL 中 Vit E含量的减少 ;显著提高脂蛋白中 SOD活性。结论　 Que和 Iso可显著抑制 Cu2 +诱导的 VL DL 的氧化修饰 ,且二者的作用相近 ,这与其抗自由基氧化活性密切相关。更多还原

【Abstract】 Objective To observe the effects of quercetin(Que) and isorhamnetin(Iso) on the oxidative modification of very low density lipoprotein(VLDL). Methods Human VLDL was prepared by one step ultracentrifuge and oxidized by Cu 2+ in vitro; Que and Iso in different concentrations were added to VLDL before incubation with Cu 2+ . The MDA, vitamin E contents and the activity of superoxide dismutase(SOD) in ox VLDL were determined. Results Compared with ox VLDL group, Que and Iso decreased MDA production in a dependent concentration manner, Que and Iso also elevated Vit E level and SOD activity markedly. Conclusion This experiment indicates that Que and Iso have similar inhibitory effects on the oxidative modification of VLDL.更多还原