【Abstract】 Venouis blood(10ml) from doped ox was treated with heparin sodium and centrifuged for 10min.A 0.5ml portion of the plasma obtained was vortexed with 1μg pipemidic acid(internal standard),0.1ml phosphate buffer of pH7.4 and 2ml CH 2Cl 2for 1min and centrifuged for 10min.The organic extract was evaporated and the residue was dissolved in 0.1ml mobile phase.Enrofloxacin(ERFX) in the solution were separated and determined by HPLC on a Shim pack CLC ODS column (15cm×6mm i.d.),operated at 36℃,with 25m M tetrabutylammonium hydroxide/methanol at pH 3(4∶1) as mobile phase at 1ml/min and detection at 272nm.The calibration graph for ERFX was linear from 0.2～3mg/l.Recoveries were 99.4% with RSD of 2.06%.The method was also used in the pharmacokinetic study of ERFX.更多还原