【摘要】 综述了化学发光、生物发光、电致化学发光中最重要的发光体系 :增强鲁米诺体系、吖啶酯发光体系、碱性磷酸酶 /1 ,2 二氧环乙烷体系、荧火虫荧光素 荧光素酶及联吡啶钌发光体系的原理 ,标记检测方法 ,最近的进展及在核酸领域的应用。对近年来新发展的发光标记方法作了介绍并分析了该领域今后可能的研究方向更多还原

【Abstract】 The principle, labeling and detection methods, characteristics, recent developments and applications especially in nucleic acid detection field of important systems of chemiluminescence, bioluminescence and electrogenerated chemiluminescence are reviewed. These systems include luminol, acridinium esters, alkaline phosphatase/ adamantyl 1,2\|dioxetane, luciferin/luciferase and tris(bipyridine)ruthenium(II). Some new techniques of probe labeling and new directions in nucleic acid analysis are given.更多还原