【摘要】 目的 :探讨Bax基因在热诱导BcaCD885细胞凋亡过程中的作用。方法 :水浴加温诱导BcaCD885细胞产生凋亡。通过FCM DNA、FCM 抗体检测及RT PCR技术 ,观测热诱导颊癌细胞凋亡过程中Bax基因蛋白及mRNA表达的动态变化。结果 :凋亡率与Bax蛋白表达水平存在正相关关系 ;加热后 ,细胞BaxmRNA相对量明显升高。结论 :高温作为一种外来刺激信号通过上调Bax基因的表达 ,诱导BcaCD885细胞凋亡的发生。更多还原

【Abstract】 Objective: This study aimed to investigate the role of Bax gene in apoptosis induced by hyperthermia in BcaCD885 cells. Methods: After inducing apoptosis through hyperthermia, the expression of Bax protein and mRNA were examined with FCM antibody analysis and RT PCR technique respectively. Results: Up regulation of Bax protein was noted after hyperthermia. The correlation coefficient r between the apoptotic rate and the expression Bax protein was 0.9240. The expression of Bax mRNA was also raised after hyperthermia. Conclusion: Hyperthermia acting as an external signal may up regulates expression of Bax gene and then induces apoptosis in BcaCD885 cells. \;更多还原